2 edition of Induction of primary anti-hapten antibodies in mice. found in the catalog.
Induction of primary anti-hapten antibodies in mice.
by Department of Bacteriology and Immunology, University of Helsinki in Helsinki
Written in English
Coulie P, Van Snick J () Enhancement of IgG anti-carrier responses by IgG2-anti-hapten antibodies in mice. Eur J Immunol – PubMed CrossRef Google Scholar Daëron M, Lesourne R () Negative signaling in Fc receptor complexes. Abstract. Central issues of current immunology are the molecular basis of antibody specificity and the origin of antibody diversity. While it is clear that antibody specificity is a consequence of the amino acid sequence of the variable regions of light and heavy polypeptide chains of the immunoglobulin molecule, for which the methodology is straight forward (by the determination of the amino.
Penicillin is a hapten in both humans and mice. To explore the hapten-carrier A primary immune response in an adult human requires approximately how much time In the immune response to a hapten-protein conjugate, in order to get anti-hapten antibodies it is essential that. A. the hapten be recognized by helper T cells. G. G. B. Klaus's 90 research works with 4, citations and 1, reads, including: CD40 regulates the processing of NF-κB2 p to p
The antibody response to a natural infection or an active immunization, however, is other words, it involves many B cells, each of which recognizes a different antigenic determinant of the immunizing antigen and secretes a different the blood serum of an immunized person or animal normally contains a mixture of antibodies, all capable of combining with the. Historically one of the major findings was that T cells and B cells are required in order to produce antibody to a complex protein. A major contribution to our understanding of this process came from studies on the formation of anti-hapten antibodies. Recall that a hapten injected by itself cannot elicit an antibody response.
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Hamaoka T, Takatsu K, Kitagawa M. Antibody production in mice. The suppressive effect of anti-carrier antibodies on cellular cooperation in the induction of secondary anti-hapten antibody responses. Immunology. Mar; 24 (3)– [PMC free article]Cited by: We studied the effect of passively administered anti-carrier and anti-hapten antibodies on the primary anti-hapten antibody response to hapten—carrier conjugates in mice.
Bacterial α-amylase (BαA), Taka-amylase A (TAA) and keyhole limpet haemocyanin (KLH) were used as carrier molecules, and 2,4-dinitrophenyl (DNP) group was used as a Cited by: 8. Abstract. Cooperative induction of secondary anti-hapten antibody responses was studied by using non-cross-reactive carrier proteins, bacterial α-amylase (BαA), Taka-amylase A (TAA) and keyhole-limpet haemocyanin (KLH), and the 2,4-dinitrophenyl (DNP) and benzylpenicilloyl (BPO) groups as Cited by: 7.
Helper activity in the anti-hapten antibody response was studied in mice in reference to the induction of delayed-type hypersensitivity (DTH) to the carrier protein. Mice were immunized either by an i.v. injection of alum-precipitated bovine serum albumin (AP-BSA) plus bacterial endotoxin or by a s.c.
injection of BSA in Freund's complete Cited by: 4. Anti-hapten antibody production was elicited by the immunization of hapten-isologous carrier conjugate (PAB-MGG) in mice. Spleen and lymph node cells taken from these primed mice could demonstrate their helper activity for anti-DNP antibody production when transferred intravenously into R X-irradiated recipient mice along with DNP-primed B cells and the double hapten conjugated Cited by: Methods are described for the efficient induction of primary anti-hapten antibody responses to trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) in vitro.
Mouse leukocytes stimulated in vitro with particulated (bentonite-adsorbed) TNP-KLH demonstrated fold more IgM-secreting, plaque-forming cells (PFC) as measured by a hemolytic plaque. Immunology Letters, 5 () Elsevier Biomedical Press THE DONOR CELL TYPE CONTROLS ANTI-HAPTEN (FLUORESCEIN ISOTHIOCYANATE) PRIMARY ANTIBODY RESPONSE TO HAPTEN-MODIFIED SYNGENEIC CELLS K.
SUZUKI, I. NAKASHIMA*, M. TAKASHI, F. NAGASE, N. KATO, K. ISOBE, K. MIZOGUCHI and M. SAITO Departments of Immunology and. Carrier-specific induction of suppression for IgG anti-hapten antibody responses Epitope-specific regulation.
Carrier-specific induction of suppression for IgG anti-hapten antibody responses. J Exp Med 1 determinants on a complex antigen. This system can be specifically induced to suppress primary and secondary IgG antibody. Induction of immunological tolerance in immunoglobulin E B lymphocytes in rats David H.
Katz, l J. Stechschulte, M.D.,* and Baruj Benacerraf, M.D. Boston, Mass. Immunological tolerance has teen induced in S,4-dw,itrophe'nyl (DNP)-specific tone marrowerived or B lymphocytes of the IgE and IgQ antibody classes by treatment of rats with the DNP derivative of the D-ammo add.
The induction of reaginic antibodies in mice by a single, intraperitoneal injection of 1 Ng DNP-OA. 3 and 4 suggest these receptors may be involved in modulating the production of reaginic antibodies during a primary immunological response. Enhancement and suppression of anti-hapten antibody formation by priming with carrier.
Immun. Accordingly, the observations that IgG responses in RAT-primed mice were induced only by TRI and not by any of the bifunctional antigens indicate that two carrier epitopes per antigen molecule are indeed required for IgG induction.
They also provide indirect evidence for synergistic help in the switching of immunoglobulin isotypes. In contrast, when antigens were specifically targeted to immature DCs in vivo, antigen presentation to CD4 + and CD8 + T cells led to profound peripheral T cell tolerance by deletion, anergy, or induction of regulatory T cells (11–14).The same antigens delivered to DCs in conjunction with a stimulus for their differentiation or maturation, such as anti-CD40 antibody (), elicited strong T.
Coulie P, Van Snick J () Enhancement of IgG anti-carrier responses by IgG2-anti-hapten antibodies in mice. Eur J Immunol – CrossRef PubMed Google Scholar Coutinho A, Forni L () The enhancement of antibody response by IgM antibodies is dependent on antigen-specific T helper cells.
Immunology is centred around the relationship between antigen and antibody. Its major practical aim is to predict what antibody activity a particular antigen will elicit, a problem which takes many forms. For example: what antibodies, either humoral or cell-bound, will be stimulated by a vaccine or allograft.
Katz DH, Paul WE, Goidl EA, Benacerraf B. Carrier function in anti-hapten immune responses. Enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization. J Exp Med. Aug 1; (2)– [PMC free.
Carrier-specific suppressor T cells caused a striking preferential loss of high affinity PFC in the primary response, but had only a slight effect on the affinity of the anti-hapten PFC formed in.
No difference in antibody titers was found between pretreated and control animals. All animals had antibodies reacting specifically to the hapten DNP, and most of them to the carrier protein BGG, whether or not delayed hypersensitivity to the carrier protein was present.
carrier function in anti-hapten immune responses i. enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization Ontogeny of B-lymphocyte function. III. Strain C57BL/6 mice produce a highly restricted primary response to the (4-hydroxynitrophenyl)acetyl (NP) group.
This response is composed of molecules having mu or gamma1 heavy chains and. Palomo C, Mas V, Thom M, et al. Influence of Respiratory Syncytial Virus F Glycoprotein Conformation on Induction of Protective Immune Responses[J].
Journal of virology,90(11): Bernardo L, Denomme G A, Shah K, et al. RhD Specific Antibodies Are Not Detectable in HLA-DRB1 Mice Challenged with Human RhD Positive Erythrocytes[J]. Cell-cell interactions in the primary antibody response B cells are not the best antigen presenting cell in a primary antibody response; dendritic cells or macrophages are more efficient.
Nevertheless, with some minor modifications the hapten-carrier model of cell-cell interactions described above also applies to interactions in a primary.After immunization of mice, each of the immunization strategies was effective for induction of IgG anti-hapten antibodies. The first immunization strategy induced a mean end-point IgG titer.The induction of an antibody response against self-antigens requires a conjugate vaccine technology, where the self-antigen is conjugated to a foreign protein sequence, and the co-application of a.